Miguel Moreno-Mateo is a Ramon and Cajal fellow and a Junior PI at CABD for approximately 3 years.
Moreno-Mateos got interested in the CRISPR technology during his post doctoral training in Antonio Giraldez’s lab at Yale University, where he met Ariel Bazzini.
In the last years, Miguel Moreno-Mateos, Ariel Bazzini and their teams worked on a project together with several groups developing mRNA knockdown technologies for their use in vertebrate organisms based on CRISPR-RfxCas13d. They observed that CRISPR-RfxCas13d knocks down maternal and zygotic mRNA in zebrafish embryos. Also that both RfxCas13d protein and mRNA can be used to recapitulate developmental phenotypes. The CRISPR-RfxCas13d is an efficient tool to interrogate embryonic gene function. This new technology is also functional in medaka, killifish and mouse embryos. The experiments of this work using medaka were performed in collaboration with Juan Ramon Martinez-Morales from CABD. The
The graphical abstract above (from Kushawah et al.) shows that CRISPR-RfxCas13d works as an efficient, specific, cost-effective, and straight forward method for the systematic and tractable study of gene function in vivo during embryogenesis in zebrafish and also across a range of animal species.
Miguel Moreno-Mateos and Ariel Bazzini on May 2019, after Bazzini’s CABD seminar.
Looking forward to see others papers from the Moreno-Mateos’ lab!