Castelli-Gair Hombria’s lab colaborated with the group of Nadia Monesi to characterize the embryonic development of B. Hygida following enzymatic treatment to permeabilize the serosal cuticle. A fruitful work recently published on Mechanisms of development.
In the figure, the enzymatic treatment reduces the amount of chitin in the serosal cuticle of B. hygida embryos. Six-day-old B. hygida embryos were processed using either the D. melanogaster
standard fixation protocol (A, B, C), or the enzymatic protocol (D, E, F), or the enzymatic
protocol in which the enzymatic step was conducted in the absence of enzyme (G, H, I). All preparations were stained with Calcofluor White. Panels (A, D, G) show fluorescence images of the same planes shown in the respective brightfield images (B, E, H). The images shown in panels (C, F, I) are three-dimensional reconstructions of confocal microscopy sections. (From Uliana et al, 2018)
The full work can be checked, here.